Multicomponent formulation for the transfection of a wide range of eukaryotic cells, for cellular analysis and protein expression.
04709705001 1.0 ml up to 300transfections 促销价RMB3800
Application
FuGENE® HDTransfection Reagent is a next-generation transfection reagent,free of animal-derived components. It is designed to transfect awide range of eukaryotic cells including insect cells and many celllines not transfected well by other reagents (e.g ., MCF-7, RAW 264.7, PC-3, HeLa, MA-10, HepG2, SH-SY5Y, A7r5, STO,SCC-61, STSAR-90, SQ20B, T98).
Note:For the most up-to-date list of celltypes that have been successfully transfected withFuGENE® HD Transfection Reagent, visit ourTransfection SpecialInterest Site at www.powerful-transfection.comand view our Transfection Reference Database. This site alsocontains much more information about the use of thisreagent. For life science researchonly.
Benefits
Generates meaningful physiological results, since FuGENE® HD Transfection Reagent has low cytotoxicity and doesn't "shock" cells (Figure 1).
Figure 3: Transfection procedure with FuGENE® HD Transfection Reagent. Please note that the procedure does not require a media change after step 2.
Generates high levels of protein expression in many adherent and suspension-adapted eukaryotic cell lines , including HEK-293 (Figure 4a), Hep G2, CHO-S, CHO-K, COS-7, COS-1, and insect cell lines such as High Five (Figure 4b) and Sf9.
Figure 4a: High transfection efficiency and protein expression in suspension-adapted mammalian cells.
Panel a: HEK-293 EBNA suspension-adapted cellswere transfected with plasmid DNA expressing GFP. The transfectionreagent / DNA ratios used were 7:2, 6:2, 5:2, 4:2, and3:2.
Panel b: The percentage of cells transfected wasdetermined 28 hours post-transfection, and the quantity of GFPprotein was estimated on a Coomassie-stained gel at 72 hourspost-transfection.
* estimated yield
Figure 4b:Hightransfection efficiency and protein expression in High Five insectcells.
Panel a: Cells were transfected with a vectorcontaining the LacZ gene. Transfection efficiency wasdemonstrated 24 hours post-transfection by histochemicalstaining for β-galactosidase activity (β-Gal StainingSet)
Panel b: Cells were transfected with vectorsexpressing human kinases Akt1 and MKK6. Protein expression in celllysates was measured by western blot at 72 hourspost-transfection. Simplifies downstreamprotein purification, since transfections with FuGENE®HD Transfection Reagent often produce high levels of expressedprotein.
Produces protein ininsect cells much faster than the baculovirus cell expressionsystem. Protein generating procedures that start withFuGENE® HD Transfection Reagent can take up to 18 fewerdays (from transfection to protein purification) than thebaclovirus system (Figure 5).
Figure 5:Comparison ofthe traditional baculovirus cell expression method with anexpression system that uses FuGENE® HD TransfectionReagent.
Product Description
Composition: unique and proprietary blend of manycomponents, in 80% ethanol.
Storage: stable at +2°C to +8°C.
Stability: 2 years, starting from date ofmanufacture.
Contents
Solution in 80%ethanol, filter-sterilized (0.1 µm filter), packaged in transparentglass vials.
Quality
Lot-specificanalysis:
Each lotof FuGENE®HD Transfection Reagent is carefully checked,using ISO DIN 9001-certified procedures, to assureproduct is performing according to specifications and is consistentfrom lot to lot.
Functionalanalysis:
Cos 7 cellsare transfected in 96 well plates with a reporter gene vector DNA,using 0.3 µl FuGENE® HD and 0.1 µg DNA or 0.25 µlFuGENE® HD and 0.1 µg DNA. Reporter gene activity ismonitored via a colorimetric test against a reference lot. Activitymust be ≥ 75% compared to a reference lot in at least one ofthe tested amounts, listed above.
Cytotoxicity analysis:
Cell growth is assessed using the Cell Proliferation Reagent WST-1assay which is anindirect measure of cytotoxicity. The remainder of cells fromfunctional analysis is tested for viability. Disclaimer
Purchaser representsand warrants that it will use FuGENE® HD Transfection Reagentpurely for research purposes. Transfected cells, materialsproduced, and any data derived from the use of FuGENE® HDTransfection Reagent, may be used only for the internal research ofPurchaser whether Purchaser is a "for-profit" or a "not-for-profit"organization. Under no circumstances may FuGENE® HD TransfectionReagent be used by Purchaser or any third party for a commercialpurpose unless Purchaser has negotiated a license for commercialuse with Fugent, LLC (contact information: License@FugentLLC.com).For purposes of the foregoing sentence, "commercial purpose" shallmean use of FuGENE® HD Transfection Reagent for profit orcommercial gain. By using FuGENE® HD Transfection Reagent,Purchaser agrees to be bound by the above terms. If Purchaserwishes not to be bound by these terms, Purchaser agrees to returnthe FuGENE® HD Transfection Reagent to Roche Diagnostics for a fullrefund